Applications of a microplate reader in yeast physiology research.
نویسندگان
چکیده
Microplate readers have been useful assistants of researchers for several decades. This work is focused on the applications of a simple absorbance microplate reader in yeast physiology research, and its advantages and limitations in comparison with alternative methods are discussed. The two main procedures involved are measuring growth curves and monitoring the pH changes of medium using two different pH indicators. We suggest mathematical formulas for converting absorbance data into pH values. With a microplate reader as many as 96 samples can be simultaneously analyzed, while medium consumption is minimized to 100 microL per sample. The results can be observed in 24-48 h (for growth curves) or in 1-3 h (for pH changes) with minimal hands-on time required.
منابع مشابه
آنالیز تأثیر امواج الکترومغناطیس بهعنوان آلودگی محیط زیست بر میزان مواد ضدسرطان گیاه ذرت با استفاده از تکنیکهای HPlC و Microplate Reader
متن کامل
Identification of the Features of E-reader Applications and Evaluation of Widely Used Iranian Applications
Purpose: The purpose of this study is to identify the features of e-reader applications through a systematic review of texts, and also to evaluate four widely used Iranian e-reader applications (Fidibo, Taghche, Ketabrah, ketab sabz) in terms of identified features. Method: The present redearch is an applied study in terms of purpose that was conducted on the basis of a systematic review frame...
متن کاملA Simple and Rapid Protocol for Producing Yeast Extract from Saccharomyces cerevisiae Suitable for Preparing Bacterial Culture Media
Yeasts, especially Saccharomyces cerevisiae, are one of the oldest organisms with broad spectrum of applications, owing to their unique genetics and physiology. Yeast extract, i.e. the product of yeast cells, is extensively used as nutritional resource in bacterial culture media. The aim of this study was to develop a simple, rapid and cost benefit process to produce the yeast extract. In this ...
متن کاملA Simple and Rapid Protocol for Producing Yeast Extract from Saccharomyces cerevisiae Suitable for Preparing Bacterial Culture Media
Yeasts, especially Saccharomyces cerevisiae, are one of the oldest organisms with broad spectrum of applications, owing to their unique genetics and physiology. Yeast extract, i.e. the product of yeast cells, is extensively used as nutritional resource in bacterial culture media. The aim of this study was to develop a simple, rapid and cost benefit process to produce the yeast extract. In this ...
متن کاملQuantitation of Cholesterol using a Fluorometric Assay and the SynergyTMHT Multi-Detection Microplate Reader
Introduction Cholesterol and Cholesterol esters are significant constituents of blood born lipoproteins. Elevated levels of these components have been linked to atherosclerosis and heart disease. As a result, a large research effort has been put into understanding cholesterol homeostasis and ways of lowering cholesterol levels. Quantitation of cholesterol in experimental samples is paramount to...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- BioTechniques
دوره 43 5 شماره
صفحات -
تاریخ انتشار 2007